Towards a systems approach for lignin biosynthesis in Populus trichocarpa: transcript abundance and specificity of the monolignol biosynthetic genes.
Identifieur interne : 003069 ( Main/Exploration ); précédent : 003068; suivant : 003070Towards a systems approach for lignin biosynthesis in Populus trichocarpa: transcript abundance and specificity of the monolignol biosynthetic genes.
Auteurs : Rui Shi [États-Unis] ; Ying-Hsuan Sun ; Quanzi Li ; Steffen Heber ; Ronald Sederoff ; Vincent L. ChiangSource :
- Plant & cell physiology [ 1471-9053 ] ; 2010.
Descripteurs français
- KwdFr :
- ARN des plantes (analyse), ARN des plantes (génétique), ARN des plantes (métabolisme), ARN messager (analyse), ARN messager (génétique), ARN messager (métabolisme), Enzymes (biosynthèse), Enzymes (génétique), Génome végétal (génétique), Lignine (biosynthèse), Lignine (génétique), Motifs d'acides aminés (génétique), Phloème (enzymologie), Phloème (génétique), Populus (génétique), Populus (métabolisme), Pousses de plante (enzymologie), Pousses de plante (génétique), Régions promotrices (génétique) (génétique), Régulation de l'expression des gènes codant pour des enzymes (physiologie), Régulation de l'expression des gènes végétaux (physiologie), Transcription génétique (physiologie), Voies de biosynthèse (génétique), Xylème (enzymologie), Xylème (génétique).
- MESH :
- analyse : ARN des plantes, ARN messager.
- biosynthèse : Enzymes, Lignine.
- enzymologie : Phloème, Pousses de plante, Xylème.
- génétique : ARN des plantes, ARN messager, Enzymes, Génome végétal, Lignine, Motifs d'acides aminés, Phloème, Populus, Pousses de plante, Régions promotrices (génétique), Voies de biosynthèse, Xylème.
- métabolisme : ARN des plantes, ARN messager, Populus.
- physiologie : Régulation de l'expression des gènes codant pour des enzymes, Régulation de l'expression des gènes végétaux, Transcription génétique.
English descriptors
- KwdEn :
- Amino Acid Motifs (genetics), Biosynthetic Pathways (genetics), Enzymes (biosynthesis), Enzymes (genetics), Gene Expression Regulation, Enzymologic (physiology), Gene Expression Regulation, Plant (physiology), Genome, Plant (genetics), Lignin (biosynthesis), Lignin (genetics), Phloem (enzymology), Phloem (genetics), Plant Shoots (enzymology), Plant Shoots (genetics), Populus (genetics), Populus (metabolism), Promoter Regions, Genetic (genetics), RNA, Messenger (analysis), RNA, Messenger (genetics), RNA, Messenger (metabolism), RNA, Plant (analysis), RNA, Plant (genetics), RNA, Plant (metabolism), Transcription, Genetic (physiology), Xylem (enzymology), Xylem (genetics).
- MESH :
- chemical , analysis : RNA, Messenger, RNA, Plant.
- chemical , biosynthesis : Enzymes, Lignin.
- enzymology : Phloem, Plant Shoots, Xylem.
- genetics : Amino Acid Motifs, Biosynthetic Pathways, Enzymes, Genome, Plant, Lignin, Phloem, Plant Shoots, Populus, Promoter Regions, Genetic, RNA, Messenger, RNA, Plant, Xylem.
- metabolism : Populus, RNA, Messenger, RNA, Plant.
- physiology : Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Transcription, Genetic.
Abstract
As a step toward a comprehensive description of lignin biosynthesis in Populus trichocarpa, we identified from the genome sequence 95 phenylpropanoid gene models in 10 protein families encoding enzymes for monolignol biosynthesis. Transcript abundance was determined for all 95 genes in xylem, leaf, shoot and phloem using quantitative real-time PCR (qRT-PCR). We identified 23 genes that most probably encode monolignol biosynthesis enzymes during wood formation. Transcripts for 18 of the 23 are abundant and specific to differentiating xylem. We found evidence suggesting functional redundancy at the transcript level for phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate:CoA ligase (4CL), p-hydroxycinnamoyl-CoA:quinate shikimate p-hydroxycinnamoyltransferase (HCT), caffeoyl-CoA O-methyltransferase (CCoAOMT) and coniferyl aldehyde 5-hydroxylase (CAld5H). We carried out an enumeration-based motif identification and discriminant analysis on the promoters of all 95 genes. Five core motifs correctly discriminate the 18 xylem-specific genes from the 77 non-xylem genes. These motifs are similar to promoter elements known to regulate phenylpropanoid gene expression. This work suggests that genes in monolignol biosynthesis are regulated by multiple motifs, often related in sequence.
DOI: 10.1093/pcp/pcp175
PubMed: 19996151
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Towards a systems approach for lignin biosynthesis in Populus trichocarpa: transcript abundance and specificity of the monolignol biosynthetic genes.</title><author><name sortKey="Shi, Rui" sort="Shi, Rui" uniqKey="Shi R" first="Rui" last="Shi">Rui Shi</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Sun, Ying Hsuan" sort="Sun, Ying Hsuan" uniqKey="Sun Y" first="Ying-Hsuan" last="Sun">Ying-Hsuan Sun</name></author><author><name sortKey="Li, Quanzi" sort="Li, Quanzi" uniqKey="Li Q" first="Quanzi" last="Li">Quanzi Li</name></author><author><name sortKey="Heber, Steffen" sort="Heber, Steffen" uniqKey="Heber S" first="Steffen" last="Heber">Steffen Heber</name></author><author><name sortKey="Sederoff, Ronald" sort="Sederoff, Ronald" uniqKey="Sederoff R" first="Ronald" last="Sederoff">Ronald Sederoff</name></author><author><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:19996151</idno><idno type="pmid">19996151</idno><idno type="doi">10.1093/pcp/pcp175</idno><idno type="wicri:Area/Main/Corpus">003370</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003370</idno><idno type="wicri:Area/Main/Curation">003370</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003370</idno><idno type="wicri:Area/Main/Exploration">003370</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Towards a systems approach for lignin biosynthesis in Populus trichocarpa: transcript abundance and specificity of the monolignol biosynthetic genes.</title><author><name sortKey="Shi, Rui" sort="Shi, Rui" uniqKey="Shi R" first="Rui" last="Shi">Rui Shi</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Sun, Ying Hsuan" sort="Sun, Ying Hsuan" uniqKey="Sun Y" first="Ying-Hsuan" last="Sun">Ying-Hsuan Sun</name></author><author><name sortKey="Li, Quanzi" sort="Li, Quanzi" uniqKey="Li Q" first="Quanzi" last="Li">Quanzi Li</name></author><author><name sortKey="Heber, Steffen" sort="Heber, Steffen" uniqKey="Heber S" first="Steffen" last="Heber">Steffen Heber</name></author><author><name sortKey="Sederoff, Ronald" sort="Sederoff, Ronald" uniqKey="Sederoff R" first="Ronald" last="Sederoff">Ronald Sederoff</name></author><author><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name></author></analytic><series><title level="j">Plant & cell physiology</title><idno type="eISSN">1471-9053</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs (genetics)</term><term>Biosynthetic Pathways (genetics)</term><term>Enzymes (biosynthesis)</term><term>Enzymes (genetics)</term><term>Gene Expression Regulation, Enzymologic (physiology)</term><term>Gene Expression Regulation, Plant (physiology)</term><term>Genome, Plant (genetics)</term><term>Lignin (biosynthesis)</term><term>Lignin (genetics)</term><term>Phloem (enzymology)</term><term>Phloem (genetics)</term><term>Plant Shoots (enzymology)</term><term>Plant Shoots (genetics)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Promoter Regions, Genetic (genetics)</term><term>RNA, Messenger (analysis)</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>RNA, Plant (analysis)</term><term>RNA, Plant (genetics)</term><term>RNA, Plant (metabolism)</term><term>Transcription, Genetic (physiology)</term><term>Xylem (enzymology)</term><term>Xylem (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (analyse)</term><term>ARN des plantes (génétique)</term><term>ARN des plantes (métabolisme)</term><term>ARN messager (analyse)</term><term>ARN messager (génétique)</term><term>ARN messager (métabolisme)</term><term>Enzymes (biosynthèse)</term><term>Enzymes (génétique)</term><term>Génome végétal (génétique)</term><term>Lignine (biosynthèse)</term><term>Lignine (génétique)</term><term>Motifs d'acides aminés (génétique)</term><term>Phloème (enzymologie)</term><term>Phloème (génétique)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Pousses de plante (enzymologie)</term><term>Pousses de plante (génétique)</term><term>Régions promotrices (génétique) (génétique)</term><term>Régulation de l'expression des gènes codant pour des enzymes (physiologie)</term><term>Régulation de l'expression des gènes végétaux (physiologie)</term><term>Transcription génétique (physiologie)</term><term>Voies de biosynthèse (génétique)</term><term>Xylème (enzymologie)</term><term>Xylème (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Messenger</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Enzymes</term><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN des plantes</term><term>ARN messager</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Enzymes</term><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phloème</term><term>Pousses de plante</term><term>Xylème</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phloem</term><term>Plant Shoots</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Amino Acid Motifs</term><term>Biosynthetic Pathways</term><term>Enzymes</term><term>Genome, Plant</term><term>Lignin</term><term>Phloem</term><term>Plant Shoots</term><term>Populus</term><term>Promoter Regions, Genetic</term><term>RNA, Messenger</term><term>RNA, Plant</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term><term>ARN messager</term><term>Enzymes</term><term>Génome végétal</term><term>Lignine</term><term>Motifs d'acides aminés</term><term>Phloème</term><term>Populus</term><term>Pousses de plante</term><term>Régions promotrices (génétique)</term><term>Voies de biosynthèse</term><term>Xylème</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term><term>RNA, Messenger</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN des plantes</term><term>ARN messager</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Régulation de l'expression des gènes végétaux</term><term>Transcription génétique</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Enzymologic</term><term>Gene Expression Regulation, Plant</term><term>Transcription, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">As a step toward a comprehensive description of lignin biosynthesis in Populus trichocarpa, we identified from the genome sequence 95 phenylpropanoid gene models in 10 protein families encoding enzymes for monolignol biosynthesis. Transcript abundance was determined for all 95 genes in xylem, leaf, shoot and phloem using quantitative real-time PCR (qRT-PCR). We identified 23 genes that most probably encode monolignol biosynthesis enzymes during wood formation. Transcripts for 18 of the 23 are abundant and specific to differentiating xylem. We found evidence suggesting functional redundancy at the transcript level for phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate:CoA ligase (4CL), p-hydroxycinnamoyl-CoA:quinate shikimate p-hydroxycinnamoyltransferase (HCT), caffeoyl-CoA O-methyltransferase (CCoAOMT) and coniferyl aldehyde 5-hydroxylase (CAld5H). We carried out an enumeration-based motif identification and discriminant analysis on the promoters of all 95 genes. Five core motifs correctly discriminate the 18 xylem-specific genes from the 77 non-xylem genes. These motifs are similar to promoter elements known to regulate phenylpropanoid gene expression. This work suggests that genes in monolignol biosynthesis are regulated by multiple motifs, often related in sequence.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19996151</PMID><DateCompleted><Year>2010</Year><Month>04</Month><Day>16</Day></DateCompleted><DateRevised><Year>2010</Year><Month>01</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1471-9053</ISSN><JournalIssue CitedMedium="Internet"><Volume>51</Volume><Issue>1</Issue><PubDate><Year>2010</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant & cell physiology</Title><ISOAbbreviation>Plant Cell Physiol</ISOAbbreviation></Journal><ArticleTitle>Towards a systems approach for lignin biosynthesis in Populus trichocarpa: transcript abundance and specificity of the monolignol biosynthetic genes.</ArticleTitle><Pagination><MedlinePgn>144-63</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/pcp/pcp175</ELocationID><Abstract><AbstractText>As a step toward a comprehensive description of lignin biosynthesis in Populus trichocarpa, we identified from the genome sequence 95 phenylpropanoid gene models in 10 protein families encoding enzymes for monolignol biosynthesis. Transcript abundance was determined for all 95 genes in xylem, leaf, shoot and phloem using quantitative real-time PCR (qRT-PCR). We identified 23 genes that most probably encode monolignol biosynthesis enzymes during wood formation. Transcripts for 18 of the 23 are abundant and specific to differentiating xylem. We found evidence suggesting functional redundancy at the transcript level for phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate:CoA ligase (4CL), p-hydroxycinnamoyl-CoA:quinate shikimate p-hydroxycinnamoyltransferase (HCT), caffeoyl-CoA O-methyltransferase (CCoAOMT) and coniferyl aldehyde 5-hydroxylase (CAld5H). We carried out an enumeration-based motif identification and discriminant analysis on the promoters of all 95 genes. Five core motifs correctly discriminate the 18 xylem-specific genes from the 77 non-xylem genes. These motifs are similar to promoter elements known to regulate phenylpropanoid gene expression. This work suggests that genes in monolignol biosynthesis are regulated by multiple motifs, often related in sequence.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Rui</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Ying-Hsuan</ForeName><Initials>YH</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Quanzi</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Heber</LastName><ForeName>Steffen</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Sederoff</LastName><ForeName>Ronald</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Chiang</LastName><ForeName>Vincent L</ForeName><Initials>VL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>12</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>Plant Cell Physiol</MedlineTA><NlmUniqueID>9430925</NlmUniqueID><ISSNLinking>0032-0781</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004798">Enzymes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020816" MajorTopicYN="N">Amino Acid Motifs</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053898" MajorTopicYN="N">Biosynthetic Pathways</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004798" MajorTopicYN="N">Enzymes</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015971" MajorTopicYN="N">Gene Expression Regulation, Enzymologic</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018745" MajorTopicYN="N">Genome, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052585" MajorTopicYN="N">Phloem</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018520" MajorTopicYN="N">Plant Shoots</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>12</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>12</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>4</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19996151</ArticleId><ArticleId IdType="pii">pcp175</ArticleId><ArticleId IdType="doi">10.1093/pcp/pcp175</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li></region></list><tree><noCountry><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name><name sortKey="Heber, Steffen" sort="Heber, Steffen" uniqKey="Heber S" first="Steffen" last="Heber">Steffen Heber</name><name sortKey="Li, Quanzi" sort="Li, Quanzi" uniqKey="Li Q" first="Quanzi" last="Li">Quanzi Li</name><name sortKey="Sederoff, Ronald" sort="Sederoff, Ronald" uniqKey="Sederoff R" first="Ronald" last="Sederoff">Ronald Sederoff</name><name sortKey="Sun, Ying Hsuan" sort="Sun, Ying Hsuan" uniqKey="Sun Y" first="Ying-Hsuan" last="Sun">Ying-Hsuan Sun</name></noCountry><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Shi, Rui" sort="Shi, Rui" uniqKey="Shi R" first="Rui" last="Shi">Rui Shi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003069 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003069 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:19996151
|texte= Towards a systems approach for lignin biosynthesis in Populus trichocarpa: transcript abundance and specificity of the monolignol biosynthetic genes.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:19996151" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |